GST pipette automatic production line - pipette use steps

　　GST pipette automatic production line manufacturer As mentioned earlier, a pipette is a measuring device used to accurately pipette a certain volume of solution. A pipette is a measuring-dispensing instrument used only to measure the volume of a solution it dispenses. It is a long, thin glass tube with an enlarged section in the middle. Its lower end is beak-shaped, and a marking line is engraved on the neck of the upper end, which is a sign of the accurate volume taken.

　　1. Introduction: Commonly used pipettes have specifications of 5, 10, 25, 50 and 75ml. The straight glass tube with scale is usually called a pipette. Commonly used pipettes are 1, 2, 5, and 10mL. Pipettes and pipettes are usually accurate to the nearest 0.01 mL.

　　2. Steps of use: Select a pipette of appropriate specifications according to the volume and requirements of the solution to be pipetted. Pipettes are generally used to accurately pipette solutions in titration analysis, and pipettes are generally used when the reaction needs to control the amount of test solution added.

　　3. Check the instrument: Check whether the nozzle and tip of the pipette are damaged. If there is any damage, it cannot be used.

　　4. Clean the instrument: rinse with tap water first, then soak in chromic acid washing solution. The operation method is as follows: hold the upper end of the pipette or pipette with the right hand at a suitable position, the index finger is close to the upper opening of the pipe, and the middle finger and ring finger are opened and held. Hold the outside of the pipette, hold the thumb on the inside of the pipette between the middle finger and the ring finger, and relax the little finger naturally; hold the ear cleaning ball in the left hand, hold the suction ear ball in the palm of the hand with the tip pointing down, and hold the suction ball tightly. Earball, to discharge the air in the ball, insert the tip of the earball into or immediately connect it to the top of the pipette (pipette), and be careful not to leak air. Slowly release the fingers of the left hand, slowly suck the washing liquid into the tube until it reaches the part above the scale line, remove the suction ear ball, quickly block the upper opening of the pipette (pipette) with the index finger of the right hand, wait for a while, and then put the washing liquid into the tube. Return the liquid to the original bottle. And rinse the inner and outer walls of the pipette (pipette) with tap water until no water drops hang, then wash with distilled water 3 times, and drain the water for later use.

　　5. Absorb the solution: Shake the solution to be absorbed, pour a small part of the solution to be absorbed into a clean and dry small beaker, use filter paper to absorb the water inside and outside the cleaned pipette tip, and insert it into the small beaker When the solution is sucked up to 1/3 of the capacity of the pipette, immediately press the mouth of the pipette with your right index finger, take it out, hold it horizontally and turn the pipette to make the solution flow all over the inner wall of the pipette, and pour the solution from the tip of the lower end Drain into the waste container. In this way, the solution can be absorbed after rinsing 3-4 times.

　　Insert the pipette that has been rinsed with the liquid to be absorbed into the place 1 to 2 cm below the surface to be absorbed, and use the suction ear ball to absorb the solution according to the above operation method (note that the pipette should not be inserted too deep into the solution, and it should be sucked down while sucking) inserted, always at this depth). When the liquid level in the tube rises to about 1-2 cm above the marked line, quickly block the nozzle with your right index finger (at this time, if the solution drops below the standard line, it should be sucked again), lift the pipette out of the liquid-absorbing surface, And make the tip of the tube touch the inner wall of the container to be sucked for a while, then lift it up, and wipe off the small amount of solution adhered to the lower end of the pipette or pipette with filter paper. (When moving the pipette or pipette, keep the pipette or pipette vertical and not tilted.)

　　6. Adjust the liquid level: Take another clean small beaker with your left hand, put the tip of the pipette against the inner wall of the small beaker, keep the small beaker tilted, keep the pipette vertical, and keep the scale line and line of sight horizontal (the left hand cannot touch the pipette. Tube). Slightly loosen the index finger (you can turn the pipette or pipette slightly), so that the solution in the tube flows out slowly from the lower mouth. When the liquid level reaches the scale line, press the right index finger firmly, pause for a moment, and then press the above method to dispense the solution. Put the bottom line of the meniscus until it is tangent to the upper edge of the marking line, and immediately press the nozzle tightly with your index finger. Put the tip close to the inner wall of the beaker, move a little towards the mouth of the beaker, and remove the droplet at the tip. Carefully transfer the pipette or pipette to the container receiving the solution.

　　7. Release the solution: put the pipette or pipette upright, the receiver is inclined, the lower end of the tube is close to the inner wall of the receiver, let go of the index finger, let the solution flow down the inner wall of the receiver, after the solution in the tube flows out, keep the discharge state and stay After 15 seconds, slide the tip of the pipette or pipette against the wall of the receiver a few times back and forth (or rotate the tip of the pipette against the inner wall of the receiver for a circle), remove the pipette (residual on the tip of the tube) A small amount of solution on the inner wall cannot be forced out by external force, because the volume of solution retained on the inner wall of the tip has been considered when calibrating pipettes or pipettes. The earball is blown out and not allowed to remain.)