Release Date:2022-12-05 16:55:52 Popularity:
The centrifugal technology of GST centrifuge tube automatic production line equipment manufacturers is mainly used for the separation and preparation of various biological samples. The suspension of biological samples is placed in a centrifuge tube under high-speed rotation. Settling at a high speed, thus being separated from the solution. Centrifuge tubes, one of the necessary experimental consumables in centrifuge experiments, vary greatly depending on their quality and performance.
1. Relative centrifugal force
A centrifuge tube has a maximum rotational speed it can withstand. When observing the working speed of the centrifuge tube, it is better to look at RCF (relative centrifugal force) instead of RPM (revolutions per minute), because RCF (relative centrifugal force) also takes gravity into account. And RPM only counts the rotational speed of the rotor. Therefore, when choosing a centrifuge tube, calculate the maximum centrifugal force you need to find a suitable centrifuge tube. If you don't need a high speed, you can choose a centrifuge tube with a relatively low centrifugal force to reduce the purchase cost.
2. Chemical compatibility
Centrifuge tubes are made of different materials, which also makes them have different resistance to different chemical substances. So the chemicals you are going to use must also be taken into consideration to ensure that the centrifuge tube you choose has good tolerance to these chemicals.
Plastic centrifuge tubes: commonly used materials such as polypropylene (PP), polyamide (PA), polycarbonate (PC), and polyethylene terephthalate (PET). Among them, the centrifuge tube made of PP polypropylene is popular because it can withstand high-speed operation, can be sterilized at high temperature, and can tolerate most organic solutions.
3. Capacity
It should be noted that when using a centrifuge tube, never fill it to the top, otherwise it will cause leakage. Fill up to 3/4 of the centrifuge tube (Note: During ultracentrifugation, the liquid in the centrifuge tube must be filled up, because ultracentrifugation requires a high vacuum, and only filling can prevent the centrifuge tube from deforming). In addition, it is also necessary to ensure that the solution in the tube is not filled too little. Only in this way can the experiment be carried out smoothly.