Freezing Tube Automatic Production Line - The correct way to use cryovials

　　Recently there have been two incidents of lyophilisation tube explosions during cell recovery in the laboratory within a week, making everyone scared when recovering cells. This time the GST Freezing Tube Automatic Production Line manufacturer will focus on how to reduce the occurrence of cell lyophilisation tube explosions and reduce the possible personal damage caused during the experimental process of cell lyophilisation and recovery.

　　Causes of lyophilization tube explosion: liquid nitrogen seeps into the lyophilization tube, when the lyophilization tube is removed from the liquid nitrogen tank, the liquid nitrogen volatilizes, resulting in rapid volume expansion and cannot be discharged in time to cause an explosion.

　　The specific reasons are: poor quality of the lyophilization tube; the lid of the lyophilization tube is not tightened, resulting in the liquid nitrogen into the tube, when removed from the liquid nitrogen tank or in the water bath heating recovery, the liquid nitrogen in the tube quickly vaporized, the volume of rapid expansion and explosion.

　　The GST automatic lyophilisation line manufacturer recommends the following solutions.

       1. lyophilisation tubes. Use imported lyophilization tubes; if you do not have any, try to use internally rotated lyophilization tubes, so that the liquid nitrogen can be easily expelled when it is vaporized compared to externally rotated lyophilization tubes, which are less likely to explode; if only externally rotated lyophilization tubes are available, then invert the lyophilization tubes as soon as possible after removal or place them horizontally for a few tens of seconds and wait for the liquid nitrogen to flow out before resuscitating the cells.

　　2. Try to add as much lyophilisation solution as possible when freezing cells, e.g. 2 ml of lyophilisation tube with 1.8 ml of lyophilisation solution, reserving a small amount of space.

　　3. Try to tighten the mouth of the lyophilisation tube when lyophilising cells.

　　4. Pre-resuscitation protection: bring canvas gloves and protective laboratory glasses and prepare forceps (to avoid direct contact with the lyophilisation tube to warm it up and to protect your hands from frostbite or frying).

　　5. When removing the cells from the liquid nitrogen, you can hold the lift rack diagonally against the upper mouth of the tank for 20 seconds before taking it out, so that the residual liquid nitrogen from the freezing cassette flows into the tank to avoid frostbite when taking it out, and to avoid an explosion when the cells are brought rapidly to room temperature from the liquid nitrogen.

　　6. After removing the lyophilisation tube: you can either put it in the freezer at -80°C and let the liquid nitrogen evaporate before resuscitating it; or move the tube from the liquid nitrogen to ice and wait for the liquid nitrogen to evaporate; or place the lyophilisation tube flat on absorbent paper or a dry towel for a few tens of seconds and wait for the liquid nitrogen to flow out naturally and evaporate until no gas comes out, then resuscitate the cells (it is difficult for the liquid nitrogen to flow out and evaporate in a short time after the lyophilisation tube is upright. When the tube is flattened, the liquid nitrogen will quickly leak out of the tube through the cracks).